RNA-Seq of Debaryomyces sp. - SRA

SRX24542932: RNA-Seq of Debaryomyces sp.
1 ILLUMINA (Illumina NovaSeq 6000) run: 33.9M spots, 10.2G bases, 5.1Gb downloads

Design: Illumina TruseqTM RNA sample prep Kit was used to construct the library. The mRNA with polyA tail was enriched by magnetic beads with Oligo(dT), and the mRNA was interrupted by ultrasound. Using fragmented mRNA as template and random oligonucleotides as primer, the first cDNA strand was synthesized in M-MuLV reverse transcriptase system, then the RNA strand was degraded by RNaseH, and the second cDNA strand was synthesized in DNA polymerase I system using dNTPs as raw material. The purified double-stranded cDNA was end-repaired, A-tail was added, and sequencing joints were connected. cDNA of about 200bp was screened by AMPure XP beads for PCR amplification, and PCR products were purified by AMPure XP beads again.

Submitted by: Nanjing agricultural university

Study: Debaryomyces sequence reads

PRJNA1068319 • SRP507527 • All experiments • All runs

show Abstracthide Abstract

Debaryomyces sp. NJAU822 was isolated from turmeric rhizome, which could convert curcumin to tetrahydrocurcumin.

Sample:

SAMN41390317 • SRS21287582 • All experiments • All runs

Organism: Debaryomyces hansenii

Library:

Name: R2

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq